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The Lab
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PCR
TC Suites
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qPCR & ddPCR Bay
1 of 2 main TC suites that contain 3 BSCs, 2 Normoxic and 2 hypoxic TC incubators. Along with every TC equipment you may need!!
Light month!!
CO2 and N2 are supplied by house provided gas and a liquid to gas interchange located in the lab. This was a custom design and buildout showing my cable management neurosis
Specialized room for off site samples before mycoplasma testing
PCR Bay
Bio-Rad CFX96
Need to keep things light!!
I know there should be anything in my BSC. "Do as I say not as I do"
Vapor LN2
Alternate View
We use Freezerworks software to label and store sample information (HIPPA)
-80 Freezer
Nikon Ti2, LED widefield, FITC/TRITC/DAPI
Lieca CM1950
-20 Freezers
Nikon Ti2, AXR, 405/488/594/759, LargeFOV, Piezo Stage, 100xSi, 40xH2O, 2x
Door to enter sterile TC suites
Nikon Ti2, LED widefield, TRITC/FITC/DAPI; Monochrome and color camera for H&E
second TC suite (this one has a great view)
Hallway for TC suites
TapeStation
TC Incubators
5K for a computer from Nikon? I built something for far less!!
Alternate View
Isofuorane Machine
Used mainly for iPSC/CRSPR colony selection (Various EVOS cubes for up to 4 colors)
Nikon Ti2, C2, 405/488/594/759, widefield FITC/TRITC/DAPI
DO NOT BUY this brand
My personal TC Suite
Luminex for Sensecent associated secratory phenotype assays
Liquid to gas interpahse for 15 Hypoxic incubators located in the Tissue Culture Suite. This custom design allowed for more working space for cell cultre specialists.
CX7 w/ onstage incubation for transendothelial migration assays
LiCOR Odyssey
Chemical Bay
Nikon Ti2, 05B Prime, CREST V2, SOLA (7ch) Intensilight, okolab incubation
NanoDrop, Plate Reader and fume hood
Leic Colony Picking microscope
My personal TC Suite
Old -90 Freezers
Bio-Rad QX200 Digital Droplet PCR
We try to make out supply corner as pleasent as possible
Bio-Rad GoGel Imager, LiCOR Odysee, Lieca CM1950
BioRad GelDoc Go
-80 Freezers and Bacterial Shakers
Nanodrop
Microscope Room
Isn't my baby beautiful!!
Leica Dissection Microscope